nedelja, 17 dec 2017



Original scientific paper

UDC: 631.466:632.95

DOI: 10.7251/afts.2014.0611.077R

COBISS.RS-ID 4573720




Radivojević Ljiljana1, Jovičić Dubravka2, Šantrić Ljiljana1, Gašić Slavica1, Umiljendić Gajić Jelena1
1Institute of Pesticides and Enviromental Protection, Belgrade, E.mail: Ova adresa el. pošte zaštićena je od spam napada, treba omogućiti JavaSkript da biste je videli
2Singidunum University, Faculty of applied ecology, Belgrad


 ABSTRACT  Full text (pdf)

Pesticide metsulfuron-methyl (chemical name: methyl 2-(4-methoxy-6-methyl-1,3,5-triazin-2-ylcarbamoylsulfamoyl)benzoate) is a post-emergence sulfonylurea herbicide which controls most annual grass broadleaf weeds in cereals, and it has been used for a long time.

The effect of metsulfuron-methyl on microbiological activity in soil was investigated. Trials were set up in laboratory on chernozem soil (pH 7.0, organic matter 3.5%, sand 26%, silt 45%, clay 29%) at Surcin, Serbia. Metsulfuron-methyl was added at rates 1, 5, 25 i 50 mg/kg soil. Untreated soil served as control. Samples were collected for analysis 5, 20, 40 and 60 days after metsulfuron-methyl application. The effects were assessed on dehydrogenase activity, bacteria abundance and fungi abundance.

Metsulfuron-methyl was found to cause different effects on the soil microbial activity in soil and its influence depended on the rate of application and duration of activity. Metsulfuron-methyl applied at 1 and 5 mg/kg soil did not have any effect on microbial parameters. The higher herbicide doses (25 and 50 mg/kg) induced increasing activity from the 5th to 40th day. These experimental data indicated that metsulfuron-methylon affected soil microbial activity, but the effects were only detected at higher doses and they were slight and transitory.


Key words: metsulfuron-methyl, dehydrogenase activity, bacteria abundance, fungi abundance,



Bridge, P., Spooner, B. (2001). Soil fingi: diversity and detection. Plant and Soil, 232, 1-2, 147-154.
Chauhan, A.K., Das, A., Kharkwal, H., Kharkwal, A.C., Varma, A. (2006). Impact of microorganisms on environmental and helth, In Microbes. Health and Environment. Chauhan A.K., Varma A. (eds), Anshan, UK, 1-12.
Sofo, A., Scopa, A., Dumontet, S., Mazzatura, A., Pasquale, V. (2012). Toxic effects of four sulphonylureas herbicides on soil microbial biomass. Journal of Environmental Science and Health, Part B: Pesticides, Food Contamination, and Agricultural Wastes, 47, 7, 653-659.
Zain, N.M.M., Rosli, B.M., Kamaruzaman, S. Morshed, M.M., Yahya, A. (2013). Effects of selected herbicides on soil microbial populations in oil palm plantation of Malaysia: A microcosm experiment. African Journal of Microbiology Research, 7, 5, 367-374.
Brown, H.M. (1990). Mode of action, crop selectivity and soil relations of the sulfonylurea herbicides. Pesticide Science, 29, 2, 263-281.
Green, J.M., Uldrich, J.F., (1993). Response of corn  (Zea mays L.) inbreds and hybrids to sulfonylurea herbicides.  Weed Science, 41, 5, 508-516.
Baghestani, M. A., Zand, E., Soufizadeh, S., Eskandari, A., Azar, R.P., Veysi., M., Nassirzadeh, N. (2007). Efficacy evaluation of some herbicides to control weeds in maize (Zea mays L.). Crop Protection, 26, 8, 936-942.
Sikkema, S.R., Soltani, N., Sikkema, P.H. and Robinson, D.E. (2008). Response of sweet maize (Zea mays L.) hybrids to halosulfuron. Crop Protection, 27, 5, 695-699, 2008.
Tomlin, C. D. S. (2011). The Pesticide Manual.Sixteenth Ed., British Crop Protection Council, Farnham, Surrey, UK.
 Dumontet, S., Peruuci, P., Scopa, A. and  Riccardi, A. (1993). Sulfonylureas: preliminary study on the effect on selected microbial strins and soil respiration. Soil Scince, 1, 1, 193-198.                                                                  
Perucci, P. and  Scarponi, L. (1996). Side effects of rimsulfuron on the microbial biomass of a clay-loam soil. Journal of  Environmental Quality, 25, 4, 610-613.
Ismail, B.S., Yapp, K.F., Omar, O. (1996). Effects of metsulfuron-metil on amylaze, urease and proteaseactivities in two soil. Australian Journal of Soil Research, 36, 3, 449-456.
Tabatabai, M. A. (1982). Soil enzymes, In Method of soil analysis, Part 2: Chemical and microbiological properties. Page, L.A., ed., American Society of Agronomy, Soil Science Society of America, Madison, Wisconsin, USA, 903-943
Sabatini, M.A., Rebecchi, L., Cappi, A., Dinelli, G., Vicari, A., Bertolani, R. (1998). Side effects of the herbicide triasulfuron on collembola under laboratory conditions. Chemosphere, 37, 8, 2963-2973.
Accinelli, C., Screpanti, C., Dinelli, G., Vicari, A. (2002). Short-time effects of pure and formulated herbicides on soil microbial activity and biomass. International Journal of Environmental Analytical Chemistry, 82, 8, 519-527.
Shaw, L.J., Burns, R.G. (2006). Enzyme activity profiles and soil quality. In Microbiological Methods for Assessing Soil Quality. Bloem, J., Hopkins, D.H., Benedetti, A. eds, CABI Publishing, Walingford, UK, 15-171
Dinelli, G., Vicari, A., Accinelli, C. (1998) Degradation and side effects of three sulfonylurea herbicides in soil. Journal of Environmental Quality, 27, 3, 1459-1464.
Radivojević, Lj., Gašić S., Šantrić, Lj., Gajić Umiljendić J., Marisavljević D. (2012). Short-time effects of herbicide nicosulfuron on the biochemical activiy on chernozem soil. Journal of the Serbian Chemical Society, 77, 6, 845-855.
Wardle, D. A., Parkinson, D. (1991). Relative importance of the effect of 2,4-D, glyphosate and environmental variables on the soil microbial biomass. Plant and Soil, 134, 209-219.
Rath, A. K., Ramarkrishnan, R. B., Rath, A. K., Kusmaraswamy, S., Bharati, K., Singla, P., Sethunathan, N. (1998). Effects of peticides on microbial biomass of flooded soil. Chemeshere, 37, 5, 661-667.
Startton, G. W., Stewart, K. E. (2002). Glyphosate effects on microbial biomass in a coniferous forest soil. Environental Toxicology Water and Quality, 7, 2, 223-236.
Radivojević, Lj., Gašić, S., Šantrić, Lj., Stanković-Kalezić, R. (2008). The impact of atrazine on several biochemical properties of chernozem soil. Journal of the Serbian Chemical Society, 73, 10, 951-959.
Ahtianen, J.H., Vanhala, P., Myllymaki, A. (2003). Effects of different plant protection programs on soil microbes. Ecotoxicology and Environmental Safety, 54, 1, 56-64.
Zabalou, M.C., Garland, J.L., Gomey, M.A. (2008). An integrated approach to evaluate the impact of the herbicides glyphosate, 2,4-D and metsulfuron-methyl on soil microbial communities in the Pampas region, Argentina, Applied Soil Ecology, 40, 1, 1-12.
Ratcliff, A.W., Busse, M.D., Shestak, C.J. (2006). Changes in microbial community structure following herbicide (glyphosate) additions to forest soils. Applied Soil Ecology, 34, 2, 114-124.
Metzger, L.O.Y., Munier-Lamy, C., Chone, T., Belgy, M.J., Andreux, F,. Vedy, J.C. (1996). Fate of a sulfonylurea herbicide in an alluvial soil, as shown by experimental degradation of pyrimidine-2-14C-labeled rimsulfuron. Chemosphere, 33, 8, 625-633.
Schneiders, G.E., Koeppe, M.K., Naidu, M.V., Horne, P., Brown, A.M., Mucha, C.F. (1993). Fate of rimsulfuron in the environment. Journal of Agricultural and Food Chemistry, 41, 7, 2404-2410.














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