×
Home Current Archive Editorial board
News Contact
Original scientific article

ESSENTIAL OIL AND EXTRACTS FROM THYMUS PRAECOX OPIZ SSP. POLYTRICHUS AS NATURAL ANTIOXIDANTS

By
V. Nada Petrović ,
V. Nada Petrović
Contact V. Nada Petrović

Faculty of Applied Ecology, Singidunum University, Belgrade, Serbia

S. Slobodan Petrović ,
S. Slobodan Petrović

BIOSS – PS and others, Belgrade, Serbia

Dubravka Jovičić ,
Dubravka Jovičić

Faculty of Applied Ecology, Singidunum University, Belgrade, Serbia

Marina Francišković
Marina Francišković

Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia

Abstract

Spices and herbs have been used for centuries by many cultures to enhance the flavor and aroma of foods. Early cultures also recognized the value of using spices and herbs in preserving foods and for their medicinal value. In the present study the essential oil of wild growing Thymus praecox Opiz ssp. polytrichus was isolated by hydrodistillationprocess. Extracts of T. praecox ssp.polytrichuswere prepared by using solvents of varying polarity. Antioxidant potential and ability of essential oil and extractsto neutralize DPPH radicals was investigated. The essential oil showed significantly higher antioxidant activity compared with synthetic antioxidants BHA and BHT. This work provides the basis for the present rapidly increasing interest for the use of natural antioxidants as food antioxidants, functional food ingredients and/or as nutritional supplements.

References

1.
Botterweck AAM, Verhagen H, Goldbohm RA, Kleinjans J, Brandt PAV. Intake of butylatedhydroxyanisole and butylatedhydroxytoluene and stomach cancer risk: results from analyses in the Netherlands cohort study. Food and Chemical Toxicology. 2000;38:599–605.
2.
E. PD, F HBJ. Natural antioxidants not exploited commercially. In: InFood Antioxidants. 1990. p. 171–92.
3.
Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of chronic disease. Critical Reviews in Food Science and Nutrition. 2004;44:275–95.
4.
Lisi A, Tedone L, Montesano V, Sarli G, Negro D. Chemical characterisation of Thymus populations belonging from Southern Italy. Food Chemistry. 2011;125:1284–6.
5.
Coelho JA, Palavra AMF. Composition and antioxidant activity of Thymus vulgaris volatiles: Comparison between supercritical fluid extraction and hydrodistillation. Journal of Separation Science. 2010;33:2211–8.
6.
Rota MC, Herrera A, Martinez RM, Sotomayor JA, Jordan MJ. Antimicrobial activity and chemical composition of Thymus vulgaris. Thymus zygis and Thymus hyemalis essential oilsFood Control. 2008;19:681–7.
7.
F. S, Stahl-Biskup E. Essential oil polymorphism in the genus Thymus. In: Thyme: The genus Thymus. 2002. p. 125–43.
8.
Morales R. The history, botany and taxonomy of the genus Thymus. In: Thyme: The genus Thymus. 2002. p. 1–44.
9.
Diklić N. Thymus L. In: Flora of the Socialistic Republic of Serbia (in Serbian), Josifović. p. 475–509.
10.
Miura K, Nakatani N. Antioxidative activity of flavonoids from thyme (Thymus vulgaris L. Agricultural and Biological Chemistry. 1989;53:3043–5.
11.
Okazaki K, Kawazoe K, Takaishi Y. Human platelet aggregation inhibitors from thyme (Thymus vulgaris L.).Phytotherapy. Research. 2002;16:398–9.
12.
Grigore A, Paraschiv I, Colceru-Mihul M, Bubueanu C, Draghici E, Ichim M. Chemical composition and antioxidant activity of Thymus vulgaris L. volatile oil obtained by two different methods. Romanian Biotechnological Letters. 2010;15:5436–43.
13.
Zarzuelo A, Crespo E. The medicinal and non-medicinal uses of thyme. In: Thyme: The genus Thymus. 2002. p. 263–92.
14.
Pharmacopeia E. 2008.
15.
A Soxhlet extraction system. 2015.
16.
Soler-Rivas C, Espin JC, Wichers HJ. An easy and fast test to compare total free radical scavenger capacity of foodstuffs.Phytochemical. Analysis. 2000;11:330–8.
17.
Sánchez-Moreno. C.(2002).Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Science and Technology International. 8:121–37.
18.
C. BD, J. H, E SB. Multivariate statistical analysis of the essential oil composition of Thymus praecoxOpiz ssp. polytrichus (Kern. ex Borb.)Ronn.collected in the Tyrolean Alps. Flavour and Fragrance Journal. 15:1–6.
19.
D. V, S. Ć, E. ŠM, M M. constituents of two rare subspecies of Thymus praecox. Natural Product Communications. 2010;5:1123–6.
20.
Kulevanova S, Ristic M, Stafilov T. The essential oils of Thymus balcanus. T ciliatopubescens and T pseudo-atticus from Macedonia, ActaPharmaceutica. 1998;48:119–26.
21.
Kačániová M, Vukovič N, Hleba L, Bobková A, Pavelková A, Rovná K, et al. and antiradicals activity of Origanumvulgare L. and Thymus vulgaris essential oils. Journal of Microbiology, Biotechnology and Food Sciences. 2012;2:263–71.
22.
Goze I, Alim A, Cetinus SA, Durmus N, Vural N, Goze HM. Chemical composition and antioxidant, antimicrobial, antispasmodic activities of the essential oil of Thymus FallaxFisch.Mey. Journal of Medicinal Plants Research. 3:174–8.
23.
Chemical composition and bioactivity stуdies of the essential oils from two Thymуs species from the Pakistani flora. LWT - Food Science and Technology. 2013;50:185–92.
24.
Nickavar B, Esbati N. Evaluation of the antioxidant capacity and phenoliccontent of three Thymus species,Journal of Acupuncture and Meridian Studies,5,119–125. 2012.
25.
M.A. A, Alghalibi SM, Alhamzy EH. Chemical composition and antimicrobial activity of essential oil of Thymus vulgaris from Yemen. Turkish Journal of Biochemistry. 2011;36:342–9.
26.
Calliste CA, Trouillas P, Allais DP, Simon A, Duroux JL. Free radical scavenging activities measured by electron spin resonance spectroscopy and B16 cell antiproliferative behaviors of seven plants. Journal of Agricultural and Food Chemistry. 2001;49:3321–7.
27.
Lee SJ, Umano K, Shibamoto T, Lee. KG. Identification of volatile components in basil (Ocimumbasilicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties. Food Chemistry. 2005;91:131–7.
28.
Yanishlieva VN, Marinova E, Pokorny J. Natural antioxidants from herbs and spices. European Journal of Lipid Science and Technology. 2006;108:776–93.
29.
Babovic N, Djilas S, Jadranin M, Vajs V, Ivanovic J, Petrovic S, et al. Supercritical carbon dioxide extraction of antioxidant fractions from selected Lamiaceae herbs and their antioxidant capacity. Innovative Food Science and Emerging Technologies. 2010;11:98–107.
30.
Babovic N, Petrović S. Antioxidants from Lamiaceae herbs. In: Food Quality: Control, Analysis and Consumer Concerns, DA. 2011. p. 311–35.

Citation

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

Article metrics

Google scholar: See link

The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.