This research paper examines the biogenic production of silver nanoparticles (AgNPs) using cyanobacterial strains (Spirulina, Nostoc, and Anabaena) that have been isolated in arid areas, Hail, in Saudi Arabia, and determines their antimicrobial effects in relation to multi-drug-resistant pathogens. Synthesis was done by incubating cyanobacterial biomass with silver nitrate in controlled conditions, and the nanoparticle was analyzed through the use of UV-Vis spectrophotometry, transmission electron microscopy (TEM), and X-ray diffraction (XRD). The agar well diffusion technique was used to determine the antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The statistical analysis (ANOVA) indicated that the cyanobacterial strain played an important role in the nanoparticle production as well as antimicrobial activity, and the F-value was 604.06 (p-value 1.21 x 10 -7). Based on the analysis of the antimicrobial activity, Nostoc-derived AgNPs had the best antimicrobial activity that was most effective in terms of the greatest zone of inhibition and lowest minimum inhibitory concentration (MIC) compared to Spirulina and Anabaena. The XRD pattern revealed that there were great variations among the crystallinity rates, with the best being the crystalline structure of Nostoc (p = 0.0258, F-value = 7.15). The MICs of AgNPs produced in Anabaena were also significantly smaller, which implies a greater antibacterial activity. Such findings present a positive indication that cyanobacterial strains, especially Nostoc, have potential in the biogenic synthesis of AgNPs with high antimicrobial qualities. The research concludes that AgNPs produced with Cyanobacterium can be used as a good and greener alternative antimicrobial agent, especially against resistant microbes, and recommends that further research be aimed at improving synthesis factors and investigating biomedical uses.
Aumpan N, Mahachai V, Vilaichone RK. Management of Helicobacter pylori infection. JGH open. 2023 Jan;7(1):3-15.
2.
Al-Ogaidi M, Al-Ogaidi I. Investigation of the antibacterial activity of Gram positive and Gram negative bacteria by 405 nm laser and nanoparticles. 2020;1136-1140.
3.
Banerjee S, Kar P, Sarkar I, Chhetri A, Mishra DK, Dutta A, Kumar A, Sinha B, Sen A. Structural elucidation and chemical characterization of underutilized fruit silverberry (Elaeagnus pyriformis) silver nanoparticles playing a dual role as anti-cancer agent by promoting apoptosis and inhibiting ABC transporters. South African Journal of Botany. 2022 Mar 1;145:243-57.
4.
Bruna T, Maldonado-Bravo F, Jara P, Caro N. Silver nanoparticles and their antibacterial applications. International journal of molecular sciences. 2021 Jul 4;22(13):7202.
5.
Campora S, Ghersi G. Recent developments and applications of smart nanoparticles in biomedicine. Nanotechnology Reviews. 2022 Jan 1;11(1):2595-631.
6.
Chopra H, Bibi S, Singh I, Hasan MM, Khan MS, Yousafi Q, Baig AA, Rahman MM, Islam F, Emran TB, Cavalu S. Green metallic nanoparticles: biosynthesis to applications. Frontiers in Bioengineering and Biotechnology. 2022 Apr 6;10:874742.
7.
Dhir R, Chauhan S, Subham P, Kumar S, Sharma P, Shidiki A, Kumar G. Plant-mediated synthesis of silver nanoparticles: unlocking their pharmacological potential–a comprehensive review. Frontiers in Bioengineering and Biotechnology. 2024 Jan 9;11:1324805.
8.
Duman H, Eker F, Akdaşçi E, Witkowska AM, Bechelany M, Karav S. Silver nanoparticles: A comprehensive review of synthesis methods and chemical and physical properties. Nanomaterials. 2024 Sep 20;14(18):1527.
9.
El-Naggar NE, Shweqa NS, Abdelmigid HM, Alyamani AA, Elshafey N, Soliman HM, Heikal YM. Mycobiosynthesis of silver nanoparticles, optimization, characterization, and in Silico anticancer activities by molecular Docking approach against hepatic and breast cancer. Biomolecules. 2024 Sep 18;14(9):1170.
10.
Fatimah I, Hidayat H, Purwiandono G, Khoirunisa K, Zahra HA, Audita R, Sagadevan S. Green synthesis of antibacterial nanocomposite of silver nanoparticle-doped hydroxyapatite utilizing Curcuma longa leaf extract and land snail (Achatina fulica) shell waste. Journal of Functional Biomaterials. 2022 Jun 20;13(2):84.
11.
Furletov AA, Apyari VV, Zaytsev VD, Sarkisyan AO, Dmitrienko SG. Silver triangular nanoplates: Synthesis and application as an analytical reagent in optical molecular spectroscopy. A review. TrAC Trends in Analytical Chemistry. 2023 Sep 1;166:117202.
12.
Godrant A, Leynaert A, Moriceau B. A study of the influence of iron, phosphate, and silicate in Si uptake by two Synechococcus strains. Frontiers in Marine Science. 2024 Jul 2;11:1331333.
13.
Gupta D, Boora A, Thakur A, Gupta TK. Green and sustainable synthesis of nanomaterials: recent advancements and limitations. Environmental Research. 2023 Aug 15;231:116316.
14.
Hada V, Chaturvedi K, Singhwane A, Siraj N, Gupta A, Sathish N, Chaurasia JP, Srivastava AK, Verma S. Nanoantibiotic effect of carbon-based nanocomposites: epicentric on graphene, carbon nanotubes and fullerene composites: a review. 3 Biotech. 2023 May;13(5):147.
15.
Hamida RS, Ali MA, Goda DA, Redhwan A. Anticandidal potential of two cyanobacteria-synthesized silver nanoparticles: Effects on growth, cell morphology, and key virulence attributes of Candida albicans. Pharmaceutics. 2021 Oct 15;13(10):1688.
16.
Javed R, Sajjad A, Naz S, Sajjad H, Ao Q. Significance of capping agents of colloidal nanoparticles from the perspective of drug and gene delivery, bioimaging, and biosensing: an insight. International Journal of Molecular Sciences. 2022 Sep 10;23(18):10521.
17.
Kavishri S, Geetha A, Ilangovar IG, Vasugi S, Sivaperumal P, Balachandran S. Facile synthesis of silver nanoparticles from sustainable Sargassum sp. seaweed material and its anti-inflammatory application. Cureus. 2024 Apr 7;16(4).
18.
Khorsandi K, Keyvani-Ghamsari S, Khatibi Shahidi F, Hosseinzadeh R, Kanwal S. A mechanistic perspective on targeting bacterial drug resistance with nanoparticles. Journal of Drug Targeting. 2021 Oct 21;29(9):941- 59.
19.
Mandhata CP, Sahoo CR, Padhy RN. Biomedical applications of biosynthesized gold nanoparticles from cyanobacteria: An overview. Biological Trace Element Research. 2022 Dec;200(12):5307-27.
20.
Mishra AK, Singh D, Deepak S, Reddy S. Modulation of Biochemical Properties in Wheat by Silver Nanoparticles Under Salt Stress Conditions. Natural and Engineering Sciences. 2025 Mar 1;10(1):244-54.
21.
Nair GM, Sajini T, Mathew B. Advanced green approaches for metal and metal oxide nanoparticles synthesis and their environmental applications. Talanta Open. 2022 Aug 1;5:100080.
22.
Phan TL, Le TT, Doan VD, Truong HA, Le VT. Size-dependent catalytic and antibacterial effects of phytogenically synthesized silver nanoparticles. Kuwait Journal of Science. 2025 Apr 1;52(2):100366.
23.
Rónavári A, Igaz N, Adamecz DI, Szerencsés B, Molnar C, Kónya Z, Pfeiffer I, Kiricsi M. Green silver and gold nanoparticles: Biological synthesis approaches and potentials for biomedical applications. Molecules. 2021 Feb 5;26(4):844.
24.
Sikes JC, Niyonshuti II, Kanokkanchana K, Chen J, Tschulik K, Fritsch I. Single particle electrochemical oxidation of polyvinylpyrrolidone-capped silver nanospheres, nanocubes, and nanoplates in potassium nitrate and potassium hydroxide solutions. Journal of The Electrochemical Society. 2022 May 9;169(5):056508.
25.
Singh H, Desimone MF, Pandya S, Jasani S, George N, Adnan M, Aldarhami A, Bazaid AS, Alderhami SA. Revisiting the green synthesis of nanoparticles: uncovering influences of plant extracts as reducing agents for enhanced synthesis efficiency and its biomedical applications. International journal of nanomedicine. 2023 Dec 31:4727-50.
26.
Saniya A, Divya R, Sharmila M, Prakash C. Anti-Diabetic And Antimicrobial Activities Of Grona Triflora Medicinal Plant. Archives for Technical Sciences/Arhiv za Tehnicke Nauke. 2025 Jan 1(32).
27.
Tripathi N, Goshisht MK. Recent advances and mechanistic insights into antibacterial activity, antibiofilm activity, and cytotoxicity of silver nanoparticles. ACS Applied Bio Materials. 2022 Mar 31;5(4):1391-463.
28.
Tejesh K, Thiru Chitrambalam M, Swarna Latha Y, Pattanaik B, Manju Bargavi SK, Kushwaha R, et al. Assessment of nano-particles for the removal of bacteria and viruses from aquatic systems. Int J Aquatic Res Environ Stud. 2025;5(1):256-261.
29.
Wang H, Nie X, You W, Huang W, Chen G, Gao F, Xia L, Zhang L, Wang L, Shen AZ, Wu KL. Tug-of-war between covalent binding and electrostatic interaction effectively killing E. coli without detectable resistance. ACS Applied Materials & Interfaces. 2021 Nov 24;13(48):56838-49.
30.
Xie W, Zhang S, Pan F, Chen S, Zhong L, Wang J, Pei X. Nanomaterial-based ROS-mediated strategies for combating bacteria and biofilms. Journal of Materials Research. 2021 Feb 28;36(4):822-45.
31.
Yusoh NA, Gill MR, Tian X. Advancing super-resolution microscopy with metal complexes: functional imaging agents for nanoscale visualization. Chemical Society Reviews. 2025.
32.
Zare EN, Zheng X, Makvandi P, Gheybi H, Sartorius R, Yiu CK, Adeli M, Wu A, Zarrabi A, Varma RS, Tay FR. Nonspherical metal‐based nanoarchitectures: synthesis and impact of size, shape, and composition on their biological activity. Small. 2021 Apr;17(17):2007073.
33.
Zare EN, Zheng X, Makvandi P, Gheybi H, Sartorius R, Yiu CK, Adeli M, Wu A, Zarrabi A, Varma RS, Tay FR. Nonspherical metal‐based nanoarchitectures: synthesis and impact of size, shape, and composition on their biological activity. Small. 2021 Apr;17(17):2007073.
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